Win, M.M.K., Aesomnuk, W., Dumhai, R. et al. (6 more authors) (2025) Transcriptomic analysis of rice cultivars with distinct resistance mechanisms to Xanthomonas oryzae pv. oryzicola reveals novel components and candidate genes associated with bacterial leaf streak. Frontiers in Plant Science, 16. 1613802. ISSN: 1664-462X
Abstract
Xanthomonas oryzae pv. oryzicola causes bacterial leaf streak, a rice disease that can lead to substantial yield losses. Although an xa5-based signaling pathway is known to confer resistance to this disease, alternative stable and broad-spectrum resistance mechanisms will be critical for sustainable management. In this regard, we characterized NDCMP49, a Thai rice variety with strong resistance to bacterial leaf streak but lacking xa5-based resistance. Transcriptomes of NDCMP49 were compared with DV85, a rice variety with xa5-based resistance, and HCS, a bacterial leaf streak susceptible variety, at 0- and 9-hours post-inoculation with X. oryzae pv. oryzicola. Analysis of differentially expressed genes revealed a less transcriptional response in the two resistant varieties than in the susceptible variety. Nonetheless, during the first nine hours of infection, all varieties showed differential expression of receptor-like kinases, NB-LRR proteins, WRKY and NAC transcription factors, heat shock proteins, and chitinases, indicating the involvement of pathogen pattern-triggered immunity and effector-triggered immunity pathways. Interestingly, genes previously associated with the Xa21-mediated resistance to closely related pathogen Xanthomonas oryzae pv. oryzae were also identified. The genotype-phenotype association analysis performed on 249 rice accessions showed that RIR1b has some InDels in the gene’s coding region, which separates the accessions according to response to X. oryzae pv. oryzicola. Moreover, 2 bp insertions in the regulatory region led to up-regulation of RIR1b in NDCMP49 over DV85 and HCS. The genes identified here are valuable candidates for functional characterization. Targeting these genes would advance breeding rice varieties with strong resistance to bacterial leaf streak and other major diseases.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2025 Win, Aesomnuk, Dumhai, Ruengphayak, Gray, Bar, Ruanjaichon, Wanchana and Arikit. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | BLS; RNA-seq; Xoc; DEGs; rice; defense response |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Date Deposited: | 29 Sep 2025 13:47 |
Last Modified: | 29 Sep 2025 13:47 |
Status: | Published |
Publisher: | Frontiers Media SA |
Refereed: | Yes |
Identification Number: | 10.3389/fpls.2025.1613802 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:232317 |