Lepeshkevich, S.V. orcid.org/0000-0001-6766-4731, Sazanovich, I.V. orcid.org/0000-0002-8000-7645, Parkhats, M.V. orcid.org/0009-0003-0378-1367 et al. (5 more authors) (2025) Direct observation of two-channel photodissociation of carbon monoxide from the hemoglobin subunits. Nature Communications, 16 (1). 7746. ISSN: 2041-1723
Abstract
Determining dynamics of bond breaking between carbon monoxide (CO) and heme proteins is essential to understand the interplay between protein function and dynamics, which is one of the fundamental challenges of physical biology. There is an ongoing debate about the mechanism of CO photodissociation from the heme iron. Here we use picosecond to millisecond transient mid-infrared spectroscopy to determine the dynamics of CO photodissociation from the isolated human hemoglobin chains. We find that the breaking of the Fe–CO bond is not a single-step process as is commonly accepted, but rather at least a two-step process, which includes both the known prompt sub-50-fs CO dissociation event and the additional slower, ~15 ps CO dissociation process discovered in this study. These results offer the direct experimental proof of the CO photodissociation mechanism containing several dissociative states.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/ licenses/by/4.0/. |
Keywords: | Biochemistry; Biological physics; Biophysical chemistry; Blood proteins; Kinetics |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Mathematical and Physical Sciences |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 27 Aug 2025 11:13 |
Last Modified: | 27 Aug 2025 11:13 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-025-63092-z |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:230809 |