Synthetic G-quadruplex components for predictable, precise two-level control of mammalian recombinant protein expression

Pohle, M., Curry, E. orcid.org/0000-0002-5123-8866, Holden, R. et al. (2 more authors) (2025) Synthetic G-quadruplex components for predictable, precise two-level control of mammalian recombinant protein expression. Nucleic Acids Research, 53 (14). gkaf732. ISSN: 0305-1048

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Item Type: Article
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©The Author(s) 2025. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords: Biochemistry and Cell Biology; Biological Sciences; Genetics; Biotechnology; Gene Therapy; G-Quadruplexes; Recombinant Proteins; Humans; Promoter Regions, Genetic; Animals; 5' Untranslated Regions; Plasmids; DNA; Genetic Vectors; RNA, Messenger; Gene Expression Regulation; HEK293 Cells
Dates:
  • Submitted: 16 September 2024
  • Accepted: 18 July 2025
  • Published (online): 30 July 2025
  • Published: 12 August 2025
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Engineering (Sheffield) > School of Chemical, Materials and Biological Engineering
Funding Information:
Funder
Grant number
Engineering and Physical Sciences Research Council
EP/T517835/1
Depositing User: Symplectic Sheffield
Date Deposited: 06 Aug 2025 15:17
Last Modified: 06 Aug 2025 15:17
Status: Published
Publisher: Oxford University Press (OUP)
Refereed: Yes
Identification Number: 10.1093/nar/gkaf732
Open Archives Initiative ID (OAI ID):

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