Ludlow, M., Povstyan, O. V., Linley, D. M. et al. (20 more authors) (2025) PIEZO1 mechanical insensitivity in generalized lymphatic dysplasia with the potential for pharmacological rescue. iScience, 28 (8). 113110. ISSN: 2589-0042
Abstract
PIEZO1 variants have been associated with generalized lymphatic dysplasia (GLD) through mechanisms involving reduced PIEZO1 expression. Here, we report variants where the mechanism involves reduced channel mechanical sensitivity. Two of the variants encode amino acid changes in the channel’s cap structure (Ile2270Thr and Arg2335Gln), one in the ninth transmembrane helical unit (THU) below the cap (Gly1978Asp) and one in the fifth THU distant from the cap (Glu829Val). Patch-clamp studies of the cap and sub-cap variant channels revealed abolished or reduced channel mechanical sensitivity with the possibility to activate the channels and partly rescue mechanical sensitivity by the small molecule Yoda1. The potency of Yoda1 at the variant channels was less than at the wild-type channel, but chemical synthesis of Yoda1 analogs revealed a molecule with improved potency. The data suggest cases of GLD in which there is decreased channel mechanical sensitivity and the potential to reduce dysfunction pharmacologically.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2025 The Author(s). This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) |
Funding Information: | Funder Grant number British Heart Foundation RG/17/11/33042 British Heart Foundation FS/4yPhD/F/21/34153 NIHR National Inst Health Research Not Known Wellcome Trust 110044/Z/15/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 10 Jul 2025 09:05 |
Last Modified: | 12 Aug 2025 13:29 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.isci.2025.113110 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:228955 |