Hooper, F.S., Revin, D.G., Theofrastou, E. et al. (5 more authors) (2025) Towards the prediction of spontaneous preterm birth by cervical collagen imaging using polarization-sensitive OCT. In: Ramella-Roman, J.C., Ma, H., Vitkin, I.A., Elson, D.S. and Novikova, T., (eds.) Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025. BiOS, 2025, 25-31 Jan 2025, San Francisco, California, United States. Proceedings of SPIE, 13322 . SPIE , p. 1332206. ISBN 9781510683921
Abstract
Introduction: Preterm birth (PTB) remains one of the leading risk factors of neonatal mortality despite the ongoing research to reduce its incidence. Spontaneous PTB (sPTB) can arise as a result of premature remodeling of the collagenrich extracellular matrix (ECM) of the cervix. Non-invasive biomarkers of cervical collagen content, such as birefringence, may thus have diagnostic value in predicting sPTB. In our previous work, we showed that polarizationsensitive OCT (PS-OCT) can characterize in vitro cervical collagen alignment through measurements of the backscattered light’s polarization state, differentiating collagen from the surrounding ECM of the cervical epithelium. To advance this towards in vivo measurements, we have developed a PS-OCT system, based on Jones Matrix calculus (JMOCT), which is an extension of PS-OCT optimized for fiber-optic beam delivery. We have coupled this to an in-house designed colposcopic probe, to characterize changes in cervical collagen organization during term and preterm pregnancies. This study aims to assess the performance of this system prior to clinical studies.
Methods: JM-OCT images of several material and biological samples with expected birefringent properties and depthbased landmarks were obtained with the colposcopic probe, including in vitro ovine cervix. OCT B-scans containing 700 A-scans were acquired with filtered phase retardance to assess the system’s resolution, imaging depth and area, and ability to present changes in sample birefringence.
Results: The system successfully produced high intensity OCT B-scans with high axial resolution (14 μm) and imaging depth (up to 2.1 mm), displaying depth-resolved landmarks including blood vessels within the in vivo skin’s papillary dermis. Changes in material and ECM birefringence were clearly displayed in phase retardance images.
Conclusion: The performance of our in-house designed colposcopic probe coupled with the in-house built JM-OCT engine shows great potential to image in vivo cervical collagen orientation and abundance in future studies. With its high axial resolution and phase retardance imaging, we aim to investigate the application of our imaging system clinically, to assess its value in understanding, predicting, and preventing PTB.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2025 The Authors. Except as otherwise noted, this author-accepted version of a paper published in Proceedings of SPIE: Polarized Light and Optical Angular Momentum for Biomedical Diagnostics 2025 is made available via the University of Sheffield Research Publications and Copyright Policy under the terms of the Creative Commons Attribution 4.0 International License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ |
Keywords: | polarization sensitive optical coherence tomography; colposcopic probe; Jones matrix calculus; preterm birth; cervical collagen |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > School of Electrical and Electronic Engineering The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine and Population Health The University of Sheffield > Faculty of Engineering (Sheffield) > School of Chemical, Materials and Biological Engineering The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Electronic and Electrical Engineering (Sheffield) |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/V010581/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 19 May 2025 16:37 |
Last Modified: | 19 May 2025 16:37 |
Status: | Published |
Publisher: | SPIE |
Series Name: | Proceedings of SPIE |
Refereed: | Yes |
Identification Number: | 10.1117/12.3041117 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:226805 |
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