Lee, L., Boorman, L., Glendenning, E. et al. (3 more authors) (Accepted: 2025) Nitric oxide is not responsible for initial sensory-induced neurovascular coupling response in the barrel cortex of lightly anaesthetised mice. Neurophotonics. ISSN 2329-423X (In Press)
Abstract
Significance: Neurovascular coupling matches changes in neural activity to localised changes in cerebral blood flow. While much is known about the role of excitatory neurons in neurovascular coupling, that of inhibitory interneurons is unresolved. While nNOS-expressing interneurons are capable of eliciting vasodilation, the role of nitric oxide in neurovascular coupling is debated.
Aim: This study investigated the role of nitric oxide in haemodynamic responses evoked by nNOS-expressing interneurons and whisker stimulation in mouse sensory cortex.
Approach: In lightly anaesthetised mice expressing channelrhodopsin-2 in nNOS- interneurons, 2D optical imaging spectroscopy was applied to measure stimulation evoked cortical haemodynamic responses. To investigate the underlying vasodilatory pathways involved, the effects of pharmacological inhibitors of NOS and 20-HETE were assessed.
Results: Haemodynamic responses evoked by nNOS-expressing interneurons were altered in the presence of the NOS inhibitor LNAME, revealing an initial 20-HETE- dependent vasoconstriction. In contrast, the initial sensory-evoked haemodynamic response was largely unchanged.
Conclusions: Our results challenge the involvement of nNOS-expressing interneurons and nitric oxide in the initiation of functional hyperemia, suggesting that nitric oxide may be involved in the recovery, rather than initiation, of sensory-induced haemodynamic responses.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2025 The Author(s). |
Keywords: | Nitric oxide; nNOS interneuron; neurovascular coupling; vasomotion; inhibitory interneuron; optogenetics |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Psychology (Sheffield) |
Funding Information: | Funder Grant number WELLCOME TRUST (THE) 105586/Z/14/Z MEDICAL RESEARCH COUNCIL MR/X003418/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 16 May 2025 15:04 |
Last Modified: | 16 May 2025 15:04 |
Status: | In Press |
Publisher: | SPIE |
Refereed: | Yes |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:226484 |
Downloads
Filename: Lee_et_al_2025_Merged_PDF.pdf

Filename: Supplementary_Material_combined_Lee_et_al_2025.pdf
