Using optical coherence tomography in plant biology research: review and prospects

Visualizing the microscopic structure of plants in vivo, non-invasively, and in real-time is the Holy Grail of botany. Optical coherence tomography (OCT) has all the characteristics necessary to achieve this feat. Indeed, OCT provides volumetric images of the internal structure of plants without the need for histological preparation. With its micrometric resolution, OCT is commonly used in medicine, primarily in ophthalmology. But it is seldom used in the field of botany. The aim of the present work is thus to review the latest technical development in the field of OCT and to highlight its current use in botany, in order to promote the technique and further advance research in the field of botany.