Cooper, R.L. orcid.org/0000-0003-0172-4708, Santos-Durán, G. and Milinkovitch, M.C. orcid.org/0000-0002-2553-0724 (2023) Protocol for the rapid intravenous in ovo injection of developing amniote embryos. STAR Protocols, 4 (2). 102324. ISSN 2666-1667
Abstract
We present a technique for precise drug delivery into the vascular system of developing amniote embryos via injection into chorioallantoic veins underlying the eggshell membrane. We describe steps for incubating and candling eggs, removing the shell to expose underlying veins, and precise intravenous injection. In addition to chicken embryos, this protocol is applicable to other amniote species that lay hard-shell eggs, including crocodiles and tortoises. This technique is rapid, is reproducible, is of low cost, and will provide an important resource for developmental biologists. For complete details on the use and execution of this protocol, please refer to Cooper & Milinkovitch.1
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Developmental Biology; Evolutionary Biology; Model Organisms |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 24 Apr 2025 10:37 |
Last Modified: | 24 Apr 2025 10:37 |
Published Version: | https://doi.org/10.1016/j.xpro.2023.102324 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.xpro.2023.102324 |
Related URLs: | |
Sustainable Development Goals: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:225727 |