Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity

Byrne, Dominic P., London, James A., Eyers, Patrick A. et al. (2 more authors) (2021) Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity. Biochemical journal. pp. 735-748. ISSN 1470-8728

Abstract

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Item Type: Article
Authors/Creators:
Copyright, Publisher and Additional Information:

© 2021 The Author(s)

Keywords: Bacterial Proteins/analysis,Bacteroides thetaiotaomicron/enzymology,Boron Compounds/analysis,Carbohydrate Conformation,Chromatography, High Pressure Liquid,Chromatography, Ion Exchange,Computer Systems,Electrophoresis, Capillary/methods,Electrophoretic Mobility Shift Assay/methods,Fluorescent Dyes/analysis,Fluorometry/methods,Glycosaminoglycans/metabolism,High-Throughput Screening Assays/methods,Kinetics,Microfluidic Analytical Techniques/methods,Nuclear Magnetic Resonance, Biomolecular,Recombinant Proteins/analysis,Substrate Specificity,Sulfotransferases/analysis
Dates:
  • Published: 18 February 2021
  • Accepted: 21 January 2021
Institution: The University of York
Academic Units: The University of York > Faculty of Sciences (York) > Biology (York)
Depositing User: Pure (York)
Date Deposited: 19 Feb 2025 16:40
Last Modified: 19 Feb 2025 17:10
Published Version: https://doi.org/10.1042/BCJ20200952
Status: Published
Refereed: Yes
Identification Number: 10.1042/BCJ20200952
Open Archives Initiative ID (OAI ID):

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Filename: bcj-2020-0952.pdf

Description: Mobility shift-based electrophoresis coupled with fluorescent detection enables real-time enzyme analysis of carbohydrate sulfatase activity

Licence: CC-BY 2.5

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