Vital-Vilchis, I. orcid.org/0000-0002-4932-9128 and Karunakaran, E. orcid.org/0000-0003-1913-4319 (2025) Make it or break it: a review on PHA synthase and depolymerase proteins. Journal of Polymers and the Environment, 33. pp. 1267-1291. ISSN 1566-2543
Abstract
Petroleum-based plastics are recalcitrant world-wide used materials that severely pollute the environment, thus biodegradable bioplastics are emerging as a viable alternative. From this group, the study of polyhydroxyalkanoates (PHAs) has stood out for their potential in diverse applications including medicine, packaging and agriculture. The enzyme responsible for PHAs synthesis inside the microbial cell is the PHA synthase (PhaC). PhaCs are present in a wide variety of microorganisms and are classified according to their substrate specificity and subunit composition into 4 classes. Class I, class III and class IV use the acyl-CoA as a precursor to synthesize short-chain-length PHAs while Class II enzymes use an intermediate of the β-oxidation pathways to synthesize medium-chain-length PHAs. Enzymes from this pathway that have been upregulated and downregulated to optimize PHAs production are described in this review. Another important enzyme is the PHA depolymerase (PhaZ) which is responsible for all PHA degradation inside and outside the cell. This review describes both enzymes in detail, including classification, structure, substrate specificity and proven protein engineering techniques for enzymatic rate enhancement and modified substrate specificity of the proteins. It also includes a mutation map for the class II PhaC sequence of Pseudomonas putida that suggest point mutations for future protein engineering work.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2025 The Authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Keywords: | Polyhydroxyalkanoates (PHAs); Bioplastics; PHA synthase (PhaC); PHA depolymerase (Z); Plastic pollution; Protein engineering |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > School of Chemical, Materials and Biological Engineering |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 20 Feb 2025 10:31 |
Last Modified: | 20 Feb 2025 10:31 |
Published Version: | https://doi.org/10.1007/s10924-024-03474-4 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1007/s10924-024-03474-4 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:223456 |