Catania, R., Heath, G.R., Rappolt, M. et al. (3 more authors) (2025) Solid-supported polymer–lipid hybrid membrane for bioelectrochemistry of a membrane redox enzyme. RSC Applied Interfaces. ISSN 2755-3701
Abstract
Hybrid membranes, consisting of phospholipids and amphiphilic block polymers, offer enhanced stability compared to liposomes and greater biocompatibility than polymersomes. These qualities make them a versatile platform for a wide range of applications across various fields. In this study, we have investigated the ability of solid-supported polymer-lipid hybrid membranes (SSHM) to act as a platform for bioelectrochemistry of membrane proteins. The redox enzyme, cytochrome bo3 (cyt bo3), a terminal oxidase in Escherichia coli, was reconstituted into hybrid vesicles (HVs), which were subsequently tested for their ability to form SSHMs on different self-assembled monolayers (SAMs) on gold electrodes. SSHM formation was monitored with electrochemical impedance spectroscopy (EIS), quartz crystal microbalance with dissipation (QCM-D), and atomic force microscopy (AFM). SSHMs were successfully formed on gold electrodes with mixed SAMs of 6-mercapto-1-hexanol and 1-hexanethiol at a 1:1 ratio. The activity of cyt bo3 was confirmed using cyclic voltammetry (CV), with electron transfer to cyt bo3 mediated by a lipophilic substrate-analogue decylubiquinone (DQ). SSHM formed with HVs-cyt bo3 samples, stored for more than one year before use, remain bioelectrocatalytically active, confirming our previously established longevity and stability of HV systems.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2025 The Author(s). This is an open access article under the terms of the Creative Commons Attribution License (CC-BY-3.0). |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Physical Chemistry (Leeds) |
Funding Information: | Funder Grant number BBSRC (Biotechnology & Biological Sciences Research Council) BB/T000546/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Feb 2025 11:29 |
Last Modified: | 06 Mar 2025 09:43 |
Status: | Published online |
Publisher: | Royal Society of Chemistry (RSC) |
Identification Number: | 10.1039/d4lf00362d |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:223427 |