Slay, E. orcid.org/0000-0003-0744-4299, Mancinelli, E., Amer, M. et al. (1 more author) (2024) Modelling and Optimization of Fluid Dynamics, Microparticles and Cell Loading in Microfluidics. In: 2024 46th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC). 2024 46th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), 15-19 Jul 2024, Orlando, FL, USA. Institute of Electrical and Electronics Engineers (IEEE) ISBN 979-8-3503-7150-5
Abstract
Organ-on-a-chip technology allows for the examination of cell cultures within dynamic systems to better understand biological pathways. The three-dimensional (3D) microenvironment in which cells reside influences their behavior and maturation via mechanobiological cues. Computational fluid dynamics can be used to model the incorporation of biomaterials and 3D constructs as well as in the spreading of cells in microfluidic devices. In our work poly(lactic acid) microparticles (MPs) are used as 3D substrate for mesenchymal stem cells (MSCs) for cancer research within the bone. Computational fluid dynamics (CFDs) was used to predict the behavior of MPs when loaded in microfluidic devices and define the optimal density for cell growth. Predicted efficiency of loading aligned with the observed MPs loaded in the microfluidic devices. A final concentration of 1,160 MPs/μL was then chosen as demonstrated to support the growth of MSCs. This work demonstrates the feasibility of using computational modelling to optimize microfluidic design and particles loading, and to assess the use of biomaterials prior to the undertaking of extensive time-consuming laboratory work in a microfluidic system.
Metadata
Item Type: | Proceedings Paper |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works. |
Keywords: | Engineered microenvironments, microfluidics, CFDs, biomaterials, tissue engineering, mesenchymal stem cells |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Electronic & Electrical Engineering (Leeds) > Pollard Institute (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Feb 2025 10:19 |
Last Modified: | 13 Feb 2025 10:19 |
Published Version: | https://ieeexplore.ieee.org/document/10782150 |
Status: | Published |
Publisher: | Institute of Electrical and Electronics Engineers (IEEE) |
Identification Number: | 10.1109/embc53108.2024.10782150 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:223222 |