Tan, S.E. orcid.org/0000-0001-7026-9900 and Strutt, D. orcid.org/0000-0001-8185-4515 (2025) Tissue shear as a cue for aligning planar polarity in the developing Drosophila wing. Nature Communications, 16 (1). 1451. ISSN 2041-1723
Abstract
Planar polarity establishment in epithelia requires interpretation of directional tissue-level information at cellular and molecular levels. Mechanical forces exerted during tissue morphogenesis are emerging as crucial tissue-level directional cues, yet the mechanisms by which they regulate planar polarity are poorly understood. Using the Drosophila pupal wing, we confirm that tissue stress promotes proximal-distal (PD) planar polarity alignment. Moreover, high tissue stress anisotropy can reduce the rate of accumulation and lower the stability on cell junctions of the core planar polarity protein Frizzled (Fz). Notably, under high tissue stress anisotropy, we see an increased gradient of cell flow, characterised by differential velocities across adjacent cell rows. This promotes core protein turnover at cell-cell contacts parallel to the flow direction, possibly via dissociation of transmembrane complexes by shear forces. We propose that gradients of cell flow play a critical role in establishing and maintaining PD-oriented polarity alignment in the developing pupal wing.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2025 The authors. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Keywords: | Body patterning; Cell polarity; Pattern formation |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 10 Feb 2025 16:59 |
Last Modified: | 10 Feb 2025 16:59 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-025-56744-7 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:223145 |