Design of an effective sgRNA for CRISPR/Cas9 knock-ins and full mutant segregation in polyploid Synechocystis sp. PCC 6803

Nares-Rodriguez, M.I. and Karunakaran, E. orcid.org/0000-0003-1913-4319 (2025) Design of an effective sgRNA for CRISPR/Cas9 knock-ins and full mutant segregation in polyploid Synechocystis sp. PCC 6803. SynBio, 3 (1). 3. ISSN 2674-0583

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Item Type: Article
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© 2025 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Keywords: CRISPR/Cas9; sgRNA design; full mutant segregation; Synechocystis sp. PCC 6803; single induction step
Dates:
  • Published: 27 January 2025
  • Published (online): 27 January 2025
  • Accepted: 27 November 2024
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Engineering (Sheffield) > School of Chemical, Materials and Biological Engineering
Depositing User: Symplectic Sheffield
Date Deposited: 11 Feb 2025 15:46
Last Modified: 11 Feb 2025 15:46
Published Version: https://doi.org/10.3390/synbio3010003
Status: Published
Publisher: MDPI AG
Refereed: Yes
Identification Number: 10.3390/synbio3010003
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Sustainable Development Goals:
  • Sustainable Development Goals: Goal 7: Affordable and Clean Energy
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