Winder, C.I., Blackburn, C., Hutchinson, C.L. et al. (3 more authors) (2024) Enzyme activity inhibition of α-amylase using Molecularly Imprinted Polymer (MIP) hydrogel microparticles. Biomacromolecules, 25 (11). pp. 7459-7465. ISSN 1525-7797
Abstract
Molecularly imprinted polymers (MIPs) are a class of synthetic recognition materials that offer a cost-effective and robust alternative to antibodies. While MIPs have found predominant use in biosensing and diagnostic applications, their potential for alternative uses, such as enzyme inhibition, remains unexplored. In this work, we synthesized a range of acrylamide-based hydrogel MIP microparticles (35 μm) specific for the recognition of α-amylase. These MIPs also showed good selectivity toward the target protein with over 96% binding of the target protein, compared with the control nonimprinted polymer (NIP) counterparts. Specificity of the MIPs was determined with the binding of nontarget proteins, trypsin, human serum albumin (HSA), and bovine serum albumin (BSA). The MIPs were further evaluated for their ability to inhibit α-amylase enzymatic activity, showing a significant decrease in activity. These findings highlight the potential of MIPs as enzyme inhibitors, suggesting an innovative application beyond their conventional use.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Authors. Except as otherwise noted, this author-accepted version of a journal article published in Biomacromolecules is made available via the University of Sheffield Research Publications and Copyright Policy under the terms of the Creative Commons Attribution 4.0 International License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ |
Keywords: | alpha-Amylases; Hydrogels; Molecularly Imprinted Polymers; Humans; Serum Albumin, Bovine; Animals; Molecular Imprinting; Enzyme Inhibitors; Cattle |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Mathematical and Physical Sciences |
Funding Information: | Funder Grant number ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/V056085/2 Engineering and Physical Sciences Research Council EP/V056085/2 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 30 Jan 2025 15:55 |
Last Modified: | 03 Feb 2025 10:25 |
Status: | Published |
Publisher: | American Chemical Society (ACS) |
Refereed: | Yes |
Identification Number: | 10.1021/acs.biomac.4c01097 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:222687 |
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Filename: AS-Enzyme_Activity_Inhibition_of_Amylase_using_Molecularly_Imprinted_Polymer.pdf
Licence: CC-BY 4.0