Cohen, Eli J, Drobnič, Tina, Ribardo, Deborah A et al. (9 more authors) (2024) Evolution of a large periplasmic disk in Campylobacterota flagella enables both efficient motility and autoagglutination. Developmental Cell. 3306-3321.e5. ISSN 1534-5807
Abstract
The flagellar motors of Campylobacter jejuni (C. jejuni) and related Campylobacterota (previously epsilonproteobacteria) feature 100-nm-wide periplasmic "basal disks" that have been implicated in scaffolding a wider ring of additional motor proteins to increase torque, but the size of these disks is excessive for a role solely in scaffolding motor proteins. Here, we show that the basal disk is a flange that braces the flagellar motor during disentanglement of its flagellar filament from interactions with the cell body and other filaments. We show that motor output is unaffected when we shrink or displace the basal disk, and suppressor mutations of debilitated motors occur in flagellar-filament or cell-surface glycosylation pathways, thus sidestepping the need for a flange to overcome the interactions between two flagellar filaments and between flagellar filaments and the cell body. Our results identify unanticipated co-dependencies in the evolution of flagellar motor structure and cell-surface properties in the Campylobacterota.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Author(s) |
Keywords: | Flagella/metabolism,Periplasm/metabolism,Campylobacter jejuni/metabolism,Bacterial Proteins/metabolism,Molecular Motor Proteins/metabolism,Agglutination |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Physics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 23 Jan 2025 16:10 |
Last Modified: | 24 Jan 2025 00:14 |
Published Version: | https://doi.org/10.1016/j.devcel.2024.09.008 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.devcel.2024.09.008 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:222273 |
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