Martinez, M.G. orcid.org/0000-0003-3183-4609, Bullock, A.J. orcid.org/0000-0003-0579-3997, MacNeil, S. orcid.org/0000-0002-2368-7955 et al. (1 more author) (2019) Characterisation of structural changes in collagen with Raman spectroscopy. Applied Spectroscopy Reviews, 54 (6). pp. 509-542. ISSN 0570-4928
Abstract
Raman spectroscopy can detect conformational changes in collagen structures and these findings are reviewed in this article. More specifically, some progressive diseases are mainly caused by alterations of collagen molecules but what is occurring at the biochemical level of this complex molecule usually remains unclear. While it may be true that a number of analytical techniques can analyze collagen, most of them have a series of limitations that limit their applicability to a wide range of samples. To understand in more detail the progression of a disease due to changes in the collagen structure, a technique that can detect subtle alterations at the biochemical level is needed. Raman spectroscopy is a label-free and noninvasive technique that can easily pick up on any conformational changes reflected primarily at the lipids, amides and proline and hydroxyproline regions. This review is the first compilation of studies of conformational changes in collagen molecules, providing help to understand changes in collagen biochemistry that can be of relevance to the human wound healing, ageing and pathologies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Taylor & Francis Group, LLC |
Keywords: | Raman spectroscopy; collagen; collagen crosslinking; secondary structure; aldehydes; lysyl residues |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > School of Chemical, Materials and Biological Engineering The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 13 Dec 2024 10:01 |
Last Modified: | 13 Dec 2024 10:01 |
Status: | Published |
Publisher: | Informa UK Limited |
Refereed: | Yes |
Identification Number: | 10.1080/05704928.2018.1506799 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:220784 |