Ma, Z., Mondor, M., Dowle, A.A. et al. (2 more authors) (2025) Buffalo worm (Alphitobius diaperinus) proteins: Structural properties, proteomics and nutritional benefits. Food Chemistry, 464 (Part 2). 141757. ISSN 0308-8146
Abstract
Biophysical methods such as circular dichroism (CD) and differential scanning calorimetry (DSC) have been minimally used to characterize insect-derived proteins. This study examines the insect Alphitobius diaperinus as a potential protein source. Techniques such as alkaline solubilization coupled to isoelectric precipitation and Osborne fractionation were used to obtain protein concentrates and fractions (albumins, globulins, prolamins, glutelins). SDS-PAGE results showed dominant protein bands at 78.3, 73.3, 49.3, 34.5, 32.0, and 10.3 kDa. All fractions had over 60 % α-helix and β-sheet structures, indicating stable conformations. Prolamins showed high surface hydrophobicity and thermal stability. Nutritionally, glutelins exhibited the highest concentration of essential amino acids (68.75 g/100 g protein), and demonstrated superior In vitro protein-digestibility (84.04 %) as well as the highest In vitro protein-digestibility corrected amino acid score (73.11 %). Therefore, this study characterized the structural-function relationship of A. diaperinus proteins and collectively assessed their suitability and safety for human consumption.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Alphitobius diaperinus; Albumins; Globulins; Prolamins; Glutelins; Protein quality |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Nov 2024 16:31 |
Last Modified: | 29 Nov 2024 16:31 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.foodchem.2024.141757 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:220237 |