Corrêa, Thamy L.R., Román, Ellen K.B., Costa, Carlos A.R. et al. (5 more authors) (2024) On the Non-Catalytic Role of Lytic Polysaccharide Monooxygenases in Boosting the Action of PETases on PET Polymers. ChemSusChem. e202401350. ISSN 1864-564X
Abstract
Synthetic polymers are resistant to biological attack, resulting in their long-term accumulation in landfills and in natural aquatic and terrestrial habitats. Lytic polysaccharide monooxygenases (LPMOs) are enzymes which oxidatively cleave the polysaccharide chains in recalcitrant polysaccharides such as cellulose. It has been widely hypothesised that LPMOs could be used to aid in the enzymatic breakdown of synthetic polymers. Herein, through the use of biochemical assays, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) we show that LPMOs can bind to polyethylene terephthalate (PET), and – in doing so – the hydrophobic surface of PET becomes more hydrophilic such that product release is boosted by subsequent treatment with classical PETases. The boosting effect is however, only observed in reactions when the LPMO and the PETase are added sequentially rather than simultaneously to the PET. Moreover, the same boosting effect is also seen when a catalytically-inactive mutant of LPMO is used, showing that the principal means by which AA9 LPMOs boost the degradation of synthetic polymers is through their role as a “hydrophobin” rather than as an oxygenase. Indeed, in accord with this role of LPMOs, we further show that this effect can be extended to other ostensibly ‘non-catalytic’ proteins beyond LPMOs, such as bovine serum albumin and lactate dehydrogenase.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Authors. ChemSusChem published by Wiley-VCH |
Keywords: | bioeconomy,LPMO,pet,PETase,plastics |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/V0040069/1 |
Depositing User: | Pure (York) |
Date Deposited: | 25 Nov 2024 09:20 |
Last Modified: | 14 Mar 2025 00:12 |
Published Version: | https://doi.org/10.1002/cssc.202401350 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1002/cssc.202401350 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:220042 |
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Description: ChemSusChem - 2024 - Corrêa - On the Non‐Catalytic Role of Lytic Polysaccharide Monooxygenases in Boosting the Action of
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