Hewitson, James Philip orcid.org/0000-0002-3265-6763, Greenman, Joanna, Moss, Lucie et al. (5 more authors) (2025) Chronic murine schistosomiasis causes aberrant hemostasis. Experimental Haematology. 104689. ISSN 0301-472X
Abstract
Background: Schistosomiasis afflicts >250 million people worldwide, leading to an annual loss of >3 million disability adjusted life years. Schistosoma mansoni causes intestinal schistosomiasis with parasite eggs either transversing intestinal tissue or lodging within the liver and other organs, causing intestinal hemorrhage and liver pathology. Large (~1cm) adult worms survive for years within blood vessels, but we lack a clear understanding of their impact on hemostasis. Objectives: We used a chronic mouse model of schistosomiasis to determine the impact on platelet numbers, phenotype and function. Methods: Hemostatic function was assessed by platelet phenotyping (flow cytometry and proteomics), whole blood aggregometry and longitudinal coagulometry. Results: Whilst platelets from schistosome-infected mice lack elevated surface P-selectin and activated αIIbβ3, unbiased proteomic analysis reveals infection-induced increases in MHC-I, IgM and IgG antibodies, and complement components. Whole blood from schistosome-infected mice spontaneously aggregates in the absence of exogenous agonists. Conversely, prothrombin and activated partial thromboplastin times are prolonged at the chronic stage of infection (10-12 weeks). Conclusions: A mouse model of S. mansoni infection shows wide-ranging changes in hemostatic function which may have clinically relevant implications for populations in endemic regions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 International Society for Experimental Hematology. Published by Elsevier Inc. |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number MEDICAL RESEARCH COUNCIL (MRC) MR/W018578/1 ACADEMY OF MEDICAL SCIENCES SBF003\1096 |
Depositing User: | Pure (York) |
Date Deposited: | 15 Nov 2024 10:50 |
Last Modified: | 02 Apr 2025 23:28 |
Published Version: | https://doi.org/10.1016/j.exphem.2024.104689 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.exphem.2024.104689 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:219695 |
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Description: Brief report: Chronic murine schistosomiasis causes aberrant hemostasis
Licence: CC-BY 2.5