Barrett, James orcid.org/0000-0003-2206-2045, Naduthodi, Mihris I S, Mao, Yuwei et al. (8 more authors) (2024) A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage. Nature Plant. ISSN 2055-0278
Abstract
CO2 fixation is commonly limited by inefficiency of the CO2-fixing enzyme Rubisco. Eukaryotic algae concentrate and fix CO2 in phase-separated condensates called pyrenoids, which complete up to one-third of global CO2 fixation. Condensation of Rubisco in pyrenoids is dependent on interaction with disordered linker proteins that show little conservation between species. We developed a sequence-independent bioinformatic pipeline to identify linker proteins in green algae. We report the linker from Chlorella and demonstrate that it binds a conserved site on the Rubisco large subunit. We show that the Chlorella linker phase separates Chlamydomonas Rubisco and that despite their separation by ~800 million years of evolution, the Chlorella linker can support the formation of a functional pyrenoid in Chlamydomonas. This cross-species reactivity extends to plants, with the Chlorella linker able to drive condensation of some native plant Rubiscos in vitro and in planta. Our results represent an exciting frontier for pyrenoid engineering in plants, which is modelled to increase crop yields.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024. The Author(s). |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Biology (York) > Centre for Novel Agricultural Products (CNAP) (York) |
Funding Information: | Funder Grant number WELLCOME TRUST 206161/Z/17/Z MEDICAL RESEARCH COUNCIL (MRC) MR/T020679/1 UKRI MR/T040742/1 |
Depositing User: | Pure (York) |
Date Deposited: | 30 Oct 2024 11:00 |
Last Modified: | 02 Apr 2025 23:28 |
Published Version: | https://doi.org/10.1038/s41477-024-01812-x |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1038/s41477-024-01812-x |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:219047 |
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Description: A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage
Licence: CC-BY 2.5