Macleod, S.-L., Super, E.H., Batt, L.J. orcid.org/0000-0001-9477-3271 et al. (2 more authors) (2024) Plate-based high-throughput fluorescence assay for assessing enveloped virus integrity. Biomacromolecules, 25 (8). pp. 4925-4933. ISSN 1525-7797
Abstract
Viruses are a considerable threat to global health and place major burdens on economies worldwide. Manufactured viruses are also being widely used as delivery agents to treat (gene therapies) or prevent diseases (vaccines). Therefore, it is vital to study and fully understand the infectious state of viruses. Current techniques used to study viruses are often slow or nonexistent, making the development of new techniques of paramount importance. Here we present a high-throughput and robust, cell-free plate-based assay (FAIRY: Fluorescence Assay for vIRal IntegritY), capable of differentiating intact from nonintact enveloped viruses, i.e, infectious from noninfectious. Using a thiazole orange-terminated polymer, a 99% increase in fluorescence was observed between treated (heat or virucide) and nontreated. The FAIRY assay allowed for the rapid determination of the infectivity of a range of enveloped viruses, highlighting its potential as a valuable tool for the study of viruses and interventions against them.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Authors. This publication is licensed under CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/). |
Keywords: | High-Throughput Screening Assays; Benzothiazoles; Fluorescence; Viruses; Quinolines; Humans; Fluorescent Dyes |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 03 Oct 2024 08:19 |
Last Modified: | 03 Oct 2024 08:19 |
Status: | Published |
Publisher: | American Chemical Society (ACS) |
Refereed: | Yes |
Identification Number: | 10.1021/acs.biomac.4c00358 |
Related URLs: | |
Sustainable Development Goals: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:217891 |
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Filename: Plate-Based High-Throughput Fluorescence Assay for Assessing Enveloped Virus Integrity.pdf
Licence: CC-BY 4.0