Xu, Z., Booth, A., Rappolt, M. et al. (3 more authors) (2024) Topological and Morphological Membrane Dynamics in Giant Lipid Vesicles Driven by Monoolein Cubosomes. Angewandte Chemie. e202414970. ISSN 0044-8249
Abstract
Lipid nanoparticles have important applications as biomedical delivery platforms and broader engineering biology applications in artificial cell technologies. These emerging technologies often require changes in the shape and topology of biological or biomimetic membranes. Here we show that topologically-active lyotropic liquid crystal nanoparticles (LCNPs) can trigger such transformations in the membranes of giant unilamellar vesicles (GUVs). Monoolein (MO) LCNPs, cubosomes with an internal nanostructure of space group Im3m incorporate into 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) GUVs creating excess membrane area with stored curvature stress. Using time-resolved fluorescence confocal and lattice light sheet microscopy, we observe and characterise various life-like dynamic events in these GUVs, including growth, division, tubulation, membrane budding and fusion. Our results shed new light on the interactions of LCNPs with bilayer lipid membranes, providing insights relevant to how these nanoparticles might interact with cellular membranes during drug delivery and highlighting their potential as minimal triggers of topological transitions in artificial cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Xu, Z., Booth, A., Rappolt, M. et al. (3 more authors) (2024) Topological and Morphological Membrane Dynamics in Giant Lipid Vesicles Driven by Monoolein Cubosomes. Angewandte Chemie, which has been published in final form at https://doi.org/10.1002/anie.202414970. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
Keywords: | Artificial cells; cubosomes; lyotropic liquid crystal nanoparticles; membrane remodelling; vesicles |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Physical Chemistry (Leeds) |
Funding Information: | Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) EP/M027929/1 EPSRC (Engineering and Physical Sciences Research Council) EP/R03608X/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 02 Oct 2024 13:31 |
Last Modified: | 02 Oct 2024 13:31 |
Status: | Published online |
Publisher: | Wiley |
Identification Number: | 10.1002/anie.202414970 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:217787 |
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