Schiffrin, R. orcid.org/0000-0002-1670-7356, Crossley, J.A. orcid.org/0000-0002-6656-1578, Walko, M. et al. (9 more authors) (2024) Dual client binding sites in the ATP-independent chaperone SurA. Nature Communications, 15. 8071. ISSN 2041-1723
Abstract
The ATP-independent chaperone SurA protects unfolded outer membrane proteins (OMPs) from aggregation in the periplasm of Gram-negative bacteria, and delivers them to the β-barrel assembly machinery (BAM) for folding into the outer membrane (OM). Precisely how SurA recognises and binds its different OMP clients remains unclear. Escherichia coli SurA comprises three domains: a core and two PPIase domains (P1 and P2). Here, by combining methyl-TROSY NMR, single-molecule Förster resonance energy transfer (smFRET), and bioinformatics analyses we show that SurA client binding is mediated by two binding hotspots in the core and P1 domains. These interactions are driven by aromatic-rich motifs in the client proteins, leading to SurA core/P1 domain rearrangements and expansion of clients from collapsed, non-native states. We demonstrate that the core domain is key to OMP expansion by SurA, and uncover a role for SurA PPIase domains in limiting the extent of expansion. The results reveal insights into SurA-OMP recognition and the mechanism of activation for an ATP-independent chaperone, and suggest a route to targeting the functions of a chaperone key to bacterial virulence and OM integrity.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2024. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 094232/Z/10/Z Wellcome Trust 094232/Z/10/Z BBSRC (Biotechnology & Biological Sciences Research Council) BB/T000635/1 Wellcome Trust 220628/Z/20/Z BBSRC (Biotechnology & Biological Sciences Research Council) BB/T008059/1 EPSRC (Engineering and Physical Sciences Research Council) EP/N013573/1 BBSRC (Biotechnology & Biological Sciences Research Council) BB/V003577/1 BBSRC (Biotechnology & Biological Sciences Research Council) BB/V003577/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Aug 2024 08:30 |
Last Modified: | 24 Jan 2025 11:16 |
Status: | Published online |
Publisher: | Nature Research |
Identification Number: | 10.1038/s41467-024-52021-1 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:216546 |