Dresser, Lara, Kunstmann, Casper, Conteduca, Donato et al. (7 more authors) (2024) Multiple Intermediates in the Detergent-Induced Fusion of Lipid Vesicles. Communications Materials. 195. ISSN 2662-4443
Abstract
Detergent-induced vesicle interactions, critical for applications including virus inactivation, varies according to the detergent type and membrane composition, but the underlying mechanistic details remain underexplored. Here, we use a lipid mixing assay based on Förster resonance energy transfer (FRET), and single-vesicle characterization approaches to identify that sub-micron-sized vesicles are induced to fuse by the non-ionic detergent Triton-X-100. We demonstrate that the process is a multi-step mechanism, characterized by discrete values of FRET efficiency between membrane-embedded fluorophores, and involves permeabilization, vesicle docking, hemi-fusion and full lipid mixing at sub-solubilizing detergent concentrations. We also dissect the kinetics of vesicle fusion to surface-tethered vesicles using a label-free quartz-crystal microbalance with dissipation monitoring approach, opening a platform for biotechnology applications. The presented strategies provide mechanistic insight into the dynamics of vesicle fusion and have implications for applications including drug delivery and sensor development where transport and manipulation of encapsulated cargo is essential.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2024 |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Physics (York) The University of York > Faculty of Sciences (York) > Biology (York) The University of York > Faculty of Sciences (York) > Electronic Engineering (York) |
Depositing User: | Pure (York) |
Date Deposited: | 21 Aug 2024 09:50 |
Last Modified: | 08 Jan 2025 00:14 |
Published Version: | https://doi.org/10.1038/s43246-024-00628-8 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1038/s43246-024-00628-8 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:216314 |
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Filename: s43246-024-00628-8.pdf
Description: Multiple intermediates in the detergent-induced fusion of lipid vesicles.
Licence: CC-BY 2.5