Marcello, E. orcid.org/0000-0002-4124-463X, Nigmatullin, R. orcid.org/0000-0003-3517-1208, Basnett, P. et al. (5 more authors) (2024) 3D melt-extrusion printing of medium chain length polyhydroxyalkanoates and their application as antibiotic-free antibacterial scaffolds for bone regeneration. ACS Biomaterials Science & Engineering, 10 (8). pp. 5136-5153. ISSN 2373-9878
Abstract
In this work, we investigated, for the first time, the possibility of developing scaffolds for bone tissue engineering through three-dimensional (3D) melt-extrusion printing of medium chain length polyhydroxyalkanoate (mcl-PHA) (i.e., poly(3-hydroxyoctanoate-co-hydroxydecanoate-co-hydroxydodecanoate), P(3HO-co-3HD-co-3HDD)). The process parameters were successfully optimized to produce well-defined and reproducible 3D P(3HO-co-3HD-co-3HDD) scaffolds, showing high cell viability (100%) toward both undifferentiated and differentiated MC3T3-E1 cells. To introduce antibacterial features in the developed scaffolds, two strategies were investigated. For the first strategy, P(3HO-co-3HD-co-3HDD) was combined with PHAs containing thioester groups in their side chains (i.e., PHACOS), inherently antibacterial PHAs. The 3D blend scaffolds were able to induce a 70% reduction of Staphylococcus aureus 6538P cells by direct contact testing, confirming their antibacterial properties. Additionally, the scaffolds were able to support the growth of MC3T3-E1 cells, showing the potential for bone regeneration. For the second strategy, composite materials were produced by the combination of P(3HO-co-3HD-co-HDD) with a novel antibacterial hydroxyapatite doped with selenium and strontium ions (Se-Sr-HA). The composite material with 10 wt % Se-Sr-HA as a filler showed high antibacterial activity against both Gram-positive (S. aureus 6538P) and Gram-negative bacteria (Escherichia coli 8739), through a dual mechanism: by direct contact (inducing 80% reduction of both bacterial strains) and through the release of active ions (leading to a 54% bacterial cell count reduction for S. aureus 6538P and 30% for E. coli 8739 after 24 h). Moreover, the composite scaffolds showed high viability of MC3T3-E1 cells through both indirect and direct testing, showing promising results for their application in bone tissue engineering.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | 3D melt-extrusion; antibacterial; bone regeneration; hydroxyapatite; polyhydroxyalkanoates; Tissue Scaffolds; Polyhydroxyalkanoates; Bone Regeneration; Animals; Mice; Anti-Bacterial Agents; Staphylococcus aureus; Printing, Three-Dimensional; Tissue Engineering; Cell Line; Cell Survival; 3T3 Cells |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Funding Information: | Funder Grant number Engineering and Physical Sciences Research Council EP/V012126/1 Engineering and Physical Sciences Research Council EP/X021440/1 ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/X026108/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 19 Aug 2024 10:18 |
Last Modified: | 19 Aug 2024 10:18 |
Published Version: | http://dx.doi.org/10.1021/acsbiomaterials.4c00624 |
Status: | Published |
Publisher: | American Chemical Society (ACS) |
Refereed: | Yes |
Identification Number: | 10.1021/acsbiomaterials.4c00624 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:216244 |