Optimisation of denaturing ion pair reversed phase HPLC for the purification of ssDNA in SELEX

Coombes, P.E. and Dickman, M.J. orcid.org/0000-0002-9236-0788 (2024) Optimisation of denaturing ion pair reversed phase HPLC for the purification of ssDNA in SELEX. Journal of Chromatography A, 1719. 464699. ISSN 0021-9673

Abstract

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Item Type: Article
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© 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Keywords: Aptamers; Ion Pair Reversed Phase HPLC; Purification; SELEX; ssDNA; Chromatography, High Pressure Liquid; SELEX Aptamer Technique; DNA, Single-Stranded; Streptavidin; Biotin; Aptamers, Nucleotide
Dates:
  • Published: 29 March 2024
  • Published (online): 2 February 2024
  • Accepted: 30 January 2024
Institution: The University of Sheffield
Academic Units: The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield)
Funding Information:
Funder
Grant number
BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL
BB/M012166/1
Depositing User: Symplectic Sheffield
Date Deposited: 08 Aug 2024 15:06
Last Modified: 08 Aug 2024 15:06
Status: Published
Publisher: Elsevier BV
Refereed: Yes
Identification Number: 10.1016/j.chroma.2024.464699
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