Chechik, Maria, Greive, Sandra Joy Muriatu orcid.org/0000-0001-6067-2632, Antson, Alfred Aksel orcid.org/0000-0002-4533-3816 et al. (1 more author) (2024) Structural basis for DNA recognition by a viral genome-packaging machine. Proceedings of the National Academy of Sciences of the United States of America. e2406138121. ISSN 1091-6490
Abstract
DNA recognition is critical for assembly of double-stranded DNA viruses, particularly for the initiation of packaging the viral genome into the capsid. The key component that recognises viral DNA is the small terminase protein. Despite prior studies, the molecular mechanism for DNA recognition remained elusive. Here we address this question by identifying the minimal site in the bacteriophage HK97 genome specifically recognised by the small terminase and determining the structure of this complex by cryoEM. The circular small terminase employs an entirely unexpected mechanism in which DNA transits through the central tunnel, and sequence-specific recognition takes place as it emerges. This recognition stems from a substructure formed by the N- and C-terminal segments of two adjacent protomers which are unstructured when DNA is absent. Such interaction ensures continuous engagement of the small terminase with DNA, enabling it to slide along the DNA while simultaneously monitoring its sequence. This mechanism allows locating and instigating packaging initiation and termination precisely at the specific cos sequence.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 the Author(s) |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Depositing User: | Pure (York) |
Date Deposited: | 17 Jul 2024 08:30 |
Last Modified: | 02 Nov 2024 01:27 |
Published Version: | https://doi.org/10.1073/pnas.2406138121 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1073/pnas.2406138121 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:214886 |
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Filename: chechik-et-al-2024-structural-basis-for-dna-recognition-by-a-viral-genome-packaging-machine.pdf
Description: Structural basis for DNA recognition by a viralgenome-packaging machine
Licence: CC-BY 2.5