Gaudioso, G., Marzorati, G., Faccenda, F. et al. (9 more authors) (2021) Processed Animal Proteins from Insect and Poultry By-Products in a Fish Meal-Free Diet for Rainbow Trout: Impact on Intestinal Microbiota and Inflammatory Markers. International Journal of Molecular Sciences, 22 (11). 5454. ISSN 1661-6596
Abstract
Sustainability of aquaculture is tied to the origin of feed ingredients. In search of sustainable fish meal‐free formulations for rainbow trout, we evaluated the effect of Hermetia illucens meal (H) and poultry by‐product meal (P), singly (10, 30, and 60% of either H or P) or in combination (10% H + 50% P, H10P50), as partial replacement of vegetable protein (VM) on gut microbiota (GM), inflammatory, and immune biomarkers. Fish fed the mixture H10P50 had the best growth performance. H, P, and especially the combination H10P50 partially restored α‐diversity that was negatively affected by VM. Diets did not differ in the Firmicutes:Proteobacteria ratio, although the relative abundance of Gammaproteobacteria was reduced in H and was higher in P and in the fishmeal control. H had higher relative abundance of chitin‐degrading Actinomyces and Bacillus, Dorea, and Enterococcus. Actinomyces was also higher in H feed, suggesting feed‐chain microbiome transmission. P increased the relative abundance of protein degraders Paeniclostridium and Bacteroidales. IL‐1β, IL‐10, TGF‐β, COX‐2, and TCR‐β gene expression in the midgut and head kidney and plasma lipopolysaccharide (LPS) revealed that the diets did not compromise the gut barrier function or induce inflammation. H, P, and H10P50 therefore appear valid protein sources in fishmeal‐free aquafeeds.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
Keywords: | rainbow trout; aquaculture; sustainability; gut microbiota; inflammation; Hermetia illucens; poultry by-products; feed-borne microbiota |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Nutrition and Public Health (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 10 Jul 2024 15:19 |
Last Modified: | 10 Jul 2024 15:19 |
Published Version: | https://www.mdpi.com/1422-0067/22/11/5454 |
Status: | Published |
Publisher: | MDPI |
Identification Number: | 10.3390/ijms22115454 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:214630 |