Schneeweiss, C., Garstka, M., Smith, J. orcid.org/0000-0002-7465-6067 et al. (2 more authors) (2009) The mechanism of action of tapasin in the peptide exchange on MHC class I molecules determined from kinetics simulation studies. Molecular Immunology, 46 (10). pp. 2054-2063. ISSN 0161-5890
Abstract
To understand the mechanism of action of the chaperone protein tapasin, which mediates loading of high-affinity peptides onto major histocompatibility complex (MHC) class I molecules in the antiviral immune response, we have performed numerical simulations of the class I-peptide binding process with four different mechanistic hypotheses from the literature, and tested our predictions by laboratory experiments. We find – in agreement of experimental and theoretical studies – that class I-peptide binding in cells is generally under kinetic control, and that tapasin introduces partial thermodynamic control to the process by competing with peptide for binding to class I. Based on our results, we suggest further experimental directions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Tapasin, Major histocompatibility complex class I molecules, Antigen presentation, Peptide binding, Receptor–ligand interaction |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 12 Jul 2024 12:52 |
Last Modified: | 12 Jul 2024 12:52 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.molimm.2009.02.032 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:214312 |