Mortazavi-Derazkola, S. orcid.org/0000-0002-1775-4525, Ebrahimzadeh, M.A., Amiri, O. et al. (4 more authors) (2020) Facile green synthesis and characterization of Crataegus microphylla extract-capped silver nanoparticles (CME@Ag-NPs) and its potential antibacterial and anticancer activities against AGS and MCF-7 human cancer cells. Journal of Alloys and Compounds, 820. 153186. ISSN 0925-8388
Abstract
A facile, fast and inexpensive method to preparation of silver nanoparticles (CME@Ag-NPs) with very favorable efficiency in biological assay has been expanded utilizing Crataegus microphylla fruit extract as reducing agent. Some parameters such as pH, time, AgNO₃ concentration and temperature on the biological assay efficiency and morphological characteristics of CME@Ag-NPs were investigated to reach optimum condition. FT-IR, XRD, TEM, DLS, FESEM, EDS and UV/Vis analysis were used to investigate the properties of the as-synthesized CME@Ag-NPs. Regular, homogeneous and spherical-like morphology of CME@Ag-NPs with a mean particle size of 30–50 nm was obtained from FESEM and TEM images. The antibacterial activity of CME@Ag-NPs was explored on Acinetobacter baumannii, Staphylococcus aureus, Enterococcus, Pseudomonas, Escherichia coli, Klebsiella and Proteus mirabilis bacteria by MIC and MBC test. Investigation of the antibacterial activity showed that as-obtained nanoparticles have significant activity against Proteus mirabilis (MIC value of 3.5 μg/ml and MBC value of 7 μg/ml) than other bacterial strains. Further, antibacterial activity of CME@Ag-NPs was determined against multidrug-resistant. Interestingly, as-synthesized silver nanoparticles displayed considerable antibacterial activity against all the test strains. In vitro cytotoxicity of nanoparticles on MCF-7 and AGS cell lines was assayed in different concentration of CME@Ag-NPs and doxorubicin as positive control. As the results show, CME@Ag-NPs is excellent effective in inhibiting the growth of MCF-7 and AGS cell lines.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Silver nanoparticles; Crataegus microphylla; Antibacterial and Anti-cancer activities |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Inorganic Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 22 Jul 2024 15:02 |
Last Modified: | 22 Jul 2024 15:02 |
Published Version: | https://www.sciencedirect.com/science/article/pii/... |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jallcom.2019.153186 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:214122 |