Shirzadi-Ahodashti, M., Mortazavi-Derazkola, S. orcid.org/0000-0002-1775-4525 and Ebrahimzadeh, M.A. (2020) Biosynthesis of noble metal nanoparticles using crataegus monogyna leaf extract (CML@X-NPs, X= Ag, Au): Antibacterial and cytotoxic activities against breast and gastric cancer cell lines. Surfaces and Interfaces, 21. 100697. ISSN 2468-0230
Abstract
In this study, eco-friendly and green synthesis of noble-metal nanoparticles using C. monogyna leaf extract (CML@X-NPs, X= Ag, Au) as capping agent and reducing agent were reported. Various conditions including pH, temperature, concentration and contact time to enhance biological assay efficiency and mean size of CML@X-NPs were studied. The as-synthesized products were characterized by TEM, FE-SEM, XRD, EDS, FT-IR and UV–Vis techniques. The uniform, spherical shape and homogeneous of CML@X-NPs with a grain size of 40–60 nm was observed from TEM and FE-SEM images. In order to determined antibacterial and antibiotic resistance activities, ATCC and multidrug-resistant isolates were identified and evaluated by micro-broth dilution method. Due to the results, the MIC values for synthesized CML@Ag-NPs and CML@Au-NPs against S. aureus, E. faecalis, P. aeruginosa, A. baumannii, E. coli, K. pneumonia and P. mirabilis were 0.29 and 750 µg/ml, 0.5 and 185 µg/ml, 0.29 and 750 µg/ml, 0.29 and 1500 µg/ml, 0.29 and 750 µg/ml, 0.5 and 370 µg/ml, 0.2 and 750 µg/ml, respectively. The cytotoxicity of the synthesized CML@Ag-NPs and CML@Au-NPs were performed using MTT assay against gastric (AGS) and breast (MCF-7) cancer cell lines. The anticancer results revealed that CML@X-NPs had significant anticancer effects against human cancer cell lines. The facile, none-toxic, fast and green synthesis of noble metal nanoparticles in presence of natural reducing agent raises their potential for biological activities like anticancer and antibacterial.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Anti-cancer, Silver nanoparticle, Crataegus monogyny, Gold nanoparticle, Antibacterial |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Inorganic Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 19 Jul 2024 14:20 |
Last Modified: | 19 Jul 2024 14:20 |
Published Version: | https://www.sciencedirect.com/science/article/pii/... |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.surfin.2020.100697 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:214119 |