Machin, Darren C, Williamson, Daniel J, Fisher, Peter et al. (11 more authors) (2024) Sortase-Modified Cholera Toxoids Show Specific Golgi Localization. Toxins. 194. ISSN 2072-6651
Abstract
Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase labeling approach to generate site-specific N-terminally modified variants of both the A2-B5 heterohexamer and B5 pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B5 pentamer showed an unexpectedly specific localization in the medial/trans-Golgi. This study suggests a future role for specifically labeled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labeling of lipid rafts in fixed cells.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 by the authors. |
Keywords: | Humans,Cholera Toxin/metabolism,Cysteine Endopeptidases/metabolism,Golgi Apparatus/metabolism,Animals,Bacterial Proteins/metabolism,Aminoacyltransferases/metabolism,Endocytosis |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 14 May 2024 09:00 |
Last Modified: | 16 Oct 2024 19:56 |
Published Version: | https://doi.org/10.3390/toxins16040194 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3390/toxins16040194 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:212511 |