Sapugahawatte, D.N. orcid.org/0000-0002-8067-8799, Godakumara, K., Mäesaar, M. orcid.org/0000-0001-5395-3233 et al. (7 more authors) (2024) Harnessing nature’s defence: the antimicrobial efficacy of pasteurised cattle milk-derived extracellular vesicles on Staphylococcus aureus ATCC 25923. International Journal of Molecular Sciences, 25 (9). p. 4759. ISSN 1661-6596
Abstract
Increasing antimicrobial resistance (AMR) challenges conventional antibiotics, prompting the search for alternatives. Extracellular vesicles (EVs) from pasteurised cattle milk offer promise, due to their unique properties. This study investigates their efficacy against five pathogenic bacteria, including Staphylococcus aureus ATCC 25923, aiming to combat AMR and to develop new therapies. EVs were characterised and tested using various methods. Co-culture experiments with S. aureus showed significant growth inhibition, with colony-forming units decreasing from 2.4 × 105 CFU/mL (single dose) to 7.4 × 104 CFU/mL (triple doses) after 12 h. Milk EVs extended lag time (6 to 9 h) and increased generation time (2.8 to 4.8 h) dose-dependently, compared to controls. In conclusion, milk EVs exhibit dose-dependent inhibition against S. aureus, prolonging lag and generation times. Despite limitations, this suggests their potential in addressing AMR.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
Keywords: | Staphylococcus aureus; extracellular vesicles; novel antimicrobials; pasteurised milk; antibacterial activity; dietary EVs |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine and Population Health |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 May 2024 11:36 |
Last Modified: | 14 May 2024 11:36 |
Status: | Published |
Publisher: | MDPI AG |
Refereed: | Yes |
Identification Number: | 10.3390/ijms25094759 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:212467 |