Garcia-Rodriguez, G. orcid.org/0000-0002-7996-7622, Girardin, Y. orcid.org/0000-0001-5765-5794, Kumar Singh, R. orcid.org/0000-0002-6929-0670 et al. (6 more authors) (2024) Toxin:antitoxin ratio sensing autoregulation of the Vibrio cholerae parDE2 module. Science Advances, 10 (1). eadj2403. ISSN 2375-2548
Abstract
The parDE family of toxin-antitoxin (TA) operons is ubiquitous in bacterial genomes and, in Vibrio cholerae, is an essential component to maintain the presence of chromosome II. Here, we show that transcription of the V. cholerae parDE2 (VcparDE) operon is regulated in a toxin:antitoxin ratio–dependent manner using a molecular mechanism distinct from other type II TA systems. The repressor of the operon is identified as an assembly with a 6:2 stoichiometry with three interacting ParD2 dimers bridged by two ParE2 monomers. This assembly docks to a three-site operator containing 5′- GGTA-3′ motifs. Saturation of this TA complex with ParE2 toxin results in disruption of the interface between ParD2 dimers and the formation of a TA complex of 2:2 stoichiometry. The latter is operator binding–incompetent as it is incompatible with the required spacing of the ParD2 dimers on the operator.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
Keywords: | Vibrio cholerae; Polymers; Antitoxins; Homeostasis; Genome, Bacterial; Operon |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Apr 2024 12:42 |
Last Modified: | 26 Apr 2024 12:42 |
Status: | Published |
Publisher: | American Association for the Advancement of Science |
Identification Number: | 10.1126/sciadv.adj2403 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:211907 |
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Filename: Toxinantitoxin ratio sensing autoregulation of the iVibrio cholerae parDE2i module.pdf
Licence: CC-BY-NC 4.0