Nalwoga, Angela, Nakibuule, Marjorie, Roshan, Romin et al. (6 more authors) (2024) Immune cell phenotype and function patterns across the life course in individuals from rural Uganda. Frontiers in immunology. p. 1356635. ISSN 1664-3224
Abstract
BACKGROUND: To determine the pattern of immune cell subsets across the life span in rural sub-Saharan Africa (SSA), and to set a reference standard for cell subsets amongst Africans, we characterised the major immune cell subsets in peripheral blood including T cells, B cells, monocytes, NK cells, neutrophils and eosinophils, in individuals aged 3 to 89 years from Uganda. METHODS: Immune phenotypes were measured using both conventional flow cytometry in 72 individuals, and full spectrum flow cytometry in 80 individuals. Epstein-Barr virus (EBV) IFN-γ T cell responses were quantified in 332 individuals using an ELISpot assay. Full blood counts of all study participants were also obtained. RESULTS: The percentages of central memory (TCM) and senescent CD4+ and CD8+ T cell subsets, effector memory (TEM) CD8+ T cells and neutrophils increased with increasing age. On the other hand, the percentages of naïve T (TN) and B (BN) cells, atypical B cells (BA), total lymphocytes, eosinophils and basophils decreased with increasing age. There was no change in CD4+ or CD8+ T effector memory RA (TEMRA) cells, exhausted T cells, NK cells and monocytes with age. Higher eosinophil and basophil percentages were observed in males compared to females. T cell function as measured by IFN-γ responses to EBV increased with increasing age, peaking at 31-55 years. CONCLUSION: The percentages of cell subsets differ between individuals from SSA compared to those elsewhere, perhaps reflecting a different antigenic milieu. These results serve as a reference for normal values in this population.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2024 Nalwoga, Nakibuule, Roshan, Kwizera Mbonye, Miley, Whitby, Newton, Rochford and Cose. |
Keywords: | Male,Female,Humans,Adult,Middle Aged,Epstein-Barr Virus Infections,Herpesvirus 4, Human,CD4-Positive T-Lymphocytes,Life Change Events,Uganda,Phenotype |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Health Sciences (York) |
Depositing User: | Pure (York) |
Date Deposited: | 16 Apr 2024 15:10 |
Last Modified: | 17 Oct 2024 08:55 |
Published Version: | https://doi.org/10.3389/fimmu.2024.1356635 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.3389/fimmu.2024.1356635 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:211565 |