Williams, R.J., Basatvat, S., Schmitz, T.C. et al. (7 more authors) (2024) Potential of notochordal cells within injectable biomaterials to promote intervertebral disc regeneration. European Cells and Materials, 47. pp. 30-50. ISSN 1473-2262
Abstract
Low back pain is the leading cause of disability worldwide and is strongly associated with degeneration of the intervertebral disc (IVD). During degeneration the nucleus pulposus (NP) in the core of the IVD, is affected by altered matrix synthesis, increased degradation, and cell loss. Strategies combining regenerative cell sources with injectable biomaterials could provide a therapeutic approach to treating IVD-degeneration related back pain. The juvenile cells of the NP, known as notochordal cells (NC), could provide both anabolic and anti-catabolic responses for disc regeneration. However, their behaviour within biomaterial delivery systems has not been investigated. Here, porcine NCs were incorporated into three injectable hydrogels: Albugel (an albumin/hyaluronan hydrogel), NPgel (a L-pNIPAM-co-DMAc hydrogel) and NPgel with decellularized NC-matrix powder (dNCM). The NCs and biomaterial constructs were cultured for up to 4 weeks under 5 % oxygen (n = 3 biological repeats). The ability of biomaterials to maintain NC viability, phenotype and extracellular matrix synthesis and deposition was investigated through histological, immunohistochemical and glycosaminoglycans analysis. NCs survived in all three biomaterials after 4 weeks, whilst phenotype and cell clustering were maintained to a greater extent in NPgel and Albugel. Thus, these biomaterials could facilitate maintenance of the NC phenotype, support matrix deposition and be a basis for future IVD regeneration strategies.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2024 The Author(s). Published by Forum Multimedia Publishing, LLC. This article is distributed in accordance with Creative Commons Attribution Licence (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | IVD degeneration; disc degeneration; biomaterial; notochordal cells; hydrogels; hyaluronic acid |
Dates: |
|
Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine and Population Health |
Funding Information: | Funder Grant number EUROPEAN COMMISSION - HORIZON 2020 825925 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 22 Mar 2024 10:43 |
Last Modified: | 22 Mar 2024 10:43 |
Status: | Published |
Publisher: | Forum Multimedia Publishing LLC |
Refereed: | Yes |
Identification Number: | 10.22203/ecm.v047a03 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:210581 |