Wan Razali, W.A. and Pandhal, J. orcid.org/0000-0002-0316-8031 (2023) Outdoor pilot-scale cultivation and techno economic assessment of a novel omega-3 eicosapentaenoic acid over-producing Nannochloropsis oculata strain. Bioresource Technology Reports, 24. 101682. ISSN 2589-014X
Abstract
Nannochloropsis oculata is a microalga that produces a significant amount of eicosapentaenoic acid (EPA). Cultivating strains in photobioreactors commercially can be challenging. We cultivated a mutant N. oculata strain, capable of accumulating >40 % EPA more than the wild-type strain at laboratory scale, in an outdoor 300 L pilot-scale photobioreactor, in the U.K. The mutant N. oculata recorded the highest amount of EPA quantity at 129.87 mg/g dry cell weight (DCW) compared to the wild-type strain, which accumulated 75.43 mg/g DCW. A techno-economic assessment (TEA) evaluated the feasibility of the study. Using specific combined cultivation scenarios, the process resulted in a positive net present value (NPV) and return on investment (ROI) at £52,156,484.46 and 607.22 % after 10 years, respectively. The TEA of the improved EPA quantity by M1 N. oculata showed the high processing cost could be overcome by high yields and an optimised operational strategy.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © Crown copyright 2023. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Microalgae; Biochemical analysis; High-value bioactive compounds; Omega-3 eicosapentaenoic acid (EPA); Mutant strain |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Funding Information: | Funder Grant number NATURAL ENVIRONMENT RESEARCH COUNCIL NE/P016820/1 BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL UNSPECIFIED ENGINEERING AND PHYSICAL SCIENCE RESEARCH COUNCIL EP/S020705/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 14 Feb 2024 14:33 |
Last Modified: | 14 Feb 2024 14:33 |
Published Version: | http://dx.doi.org/10.1016/j.biteb.2023.101682 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.biteb.2023.101682 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:208925 |