Sullivan, M.V. orcid.org/0000-0002-1771-8268, Clay, O., Moazami, M.P. et al. (2 more authors) (2021) Hybrid aptamer‐molecularly imprinted polymer (aptaMIP) nanoparticles from protein recognition—a trypsin model. Macromolecular Bioscience, 21 (5). 2100002. ISSN 1616-5187
Abstract
Aptamers offer excellent potential for replacing antibodies for molecular recognition purposes however their performance can compromise with biological/environmental degradation being a particular problem. Molecularly imprinted Polymers (MIPs) offer an alternative to biological materials and while these offer the robustness and ability to work in extreme environmental conditions, they often lack the same recognition performance. By slightly adapting the chemical structure of a DNA aptamer it is incorporated for use as the recognition part of a MIP, thus creating an aptamer-MIP hybrid or aptaMIP. Here these are developed for the detection of the target protein trypsin. The aptaMIP nanoparticles offer superior binding affinity over conventional MIP nanoparticles (nanoMIPs), with KD values of 6.8 × 10−9 (±0.2 × 10−9) m and 12.3 × 10−9 (±0.4 × 10−9) m for the aptaMIP and nanoMIP, respectively. The aptaMIP also outperforms the aptamer only (10.3 × 10−9 m). Good selectivity against other protein targets is observed. Using surface plasmon resonance, the limit of detection for aptaMIP nanoparticles is twofold lower (2 nm) compared to the nanoMIP (4 nm). Introduction of the aptamer as a “macro-monomer” into the MIP scaffold has beneficial effects and offers potential to improve this class of polymers significantly.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ |
Keywords: | aptamers; molecularly imprinted polymers; nanoparticles; protein; solid-phase synthesis; Aptamers, Nucleotide; Biosensing Techniques; Models, Molecular; Molecularly Imprinted Polymers; Muramidase; Nanoparticles; Serum Albumin, Bovine; Surface Plasmon Resonance; Trypsin |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > Department of Chemistry (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 31 Jan 2024 14:50 |
Last Modified: | 31 Jan 2024 14:50 |
Status: | Published |
Publisher: | Wiley |
Refereed: | Yes |
Identification Number: | 10.1002/mabi.202100002 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:208455 |