Cooper, F. orcid.org/0000-0002-5340-619X and Tsakiridis, A. orcid.org/0000-0002-2184-2990 (2022) Towards clinical applications of in vitro-derived axial progenitors. Developmental Biology, 489. pp. 110-117. ISSN 0012-1606
Abstract
The production of the tissues that make up the mammalian embryonic trunk takes place in a head-tail direction, via the differentiation of posteriorly-located axial progenitor populations. These include bipotent neuromesodermal progenitors (NMPs), which generate both spinal cord neurectoderm and presomitic mesoderm, the precursor of the musculoskeleton. Over the past few years, a number of studies have described the derivation of NMP-like cells from mouse and human pluripotent stem cells (PSCs). In turn, these have greatly facilitated the establishment of PSC differentiation protocols aiming to give rise efficiently to posterior mesodermal and neural cell types, which have been particularly challenging to produce using previous approaches. Moreover, the advent of 3-dimensional-based culture systems incorporating distinct axial progenitor-derived cell lineages has opened new avenues toward the functional dissection of early patterning events and cell vs non-cell autonomous effects. Here, we provide a brief overview of the applications of these cell types in disease modelling and cell therapy and speculate on their potential uses in the future.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Axis elongation; Cell therapy; Disease modelling; Neuromesodermal progenitors; Animals; Body Patterning; Cell Differentiation; Cell Lineage; Humans; Mammals; Mesoderm; Mice; Neural Stem Cells |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL BB/P000444/1 MEDICAL RESEARCH COUNCIL MR/V002163/1 Children's Cancer and Leukaemia Group CCLGA 2019 28 EUROPEAN COMMISSION - HORIZON 2020 824070 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 26 Jan 2024 16:40 |
Last Modified: | 26 Jan 2024 16:40 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.ydbio.2022.06.006 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:207898 |