Bellantuono, I. orcid.org/0000-0001-9994-6987, de Cabo, R., Ehninger, D. et al. (9 more authors) (2020) A toolbox for the longitudinal assessment of healthspan in aging mice. Nature Protocols, 15 (2). pp. 540-574. ISSN 1754-2189
Abstract
The number of people aged over 65 is expected to double in the next 30 years. For many, living longer will mean spending more years with the burdens of chronic diseases such as Alzheimer’s disease, cardiovascular disease, and diabetes. Although researchers have made rapid progress in developing geroprotective interventions that target mechanisms of aging and delay or prevent the onset of multiple concurrent age-related diseases, a lack of standardized techniques to assess healthspan in preclinical murine studies has resulted in reduced reproducibility and slow progress. To overcome this, major centers in Europe and the United States skilled in healthspan analysis came together to agree on a toolbox of techniques that can be used to consistently assess the healthspan of mice. Here, we describe the agreed toolbox, which contains protocols for echocardiography, novel object recognition, grip strength, rotarod, glucose tolerance test (GTT) and insulin tolerance test (ITT), body composition, and energy expenditure. The protocols can be performed longitudinally in the same mouse over a period of 4–6 weeks to test how candidate geroprotectors affect cardiac, cognitive, neuromuscular, and metabolic health.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020, Springer Nature. This is an author-produced version of a paper subsequently published in nature protocols. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Aging; Animals; Body Composition; Electrocardiography; Energy Metabolism; Glucose Tolerance Test; Hand Strength; Health; Insulin Resistance; Longitudinal Studies; Mice; Mice, Inbred C57BL; Recognition, Psychology |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine and Population Health |
Funding Information: | Funder Grant number EUROPEAN COMMISSION - HORIZON 2020 730879 MEDICAL RESEARCH COUNCIL MR/P020941/1 BRITISH HEART FOUNDATION FS/18/52/33808 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 23 Jan 2024 11:40 |
Last Modified: | 23 Jan 2024 11:40 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1038/s41596-019-0256-1 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:207850 |