Andrews, P.W. orcid.org/0000-0001-7215-4410 and Gokhale, P.J. orcid.org/0000-0001-7225-4403 (2024) A short history of pluripotent stem cells markers. Stem Cell Reports, 19 (1). pp. 1-10. ISSN 2213-6711
Abstract
The expression of one or more of a small number of molecules, typically cell surface-associated antigens, or transcription factors, is widely used for identifying pluripotent stem cells (PSCs) or for monitoring their differentiation. However, none of these marker molecules are uniquely expressed by PSCs and all are expressed by stem cells that have lost the ability to differentiate. Consequently, none are indicators of pluripotency, per se. Here we summarize the nature and characteristics of several markers that are in wide use, including the cell surface antigens, stage-specific embryonic antigen (SSEA)-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81, GCTM2, and the transcription factors POUF5/OCT4, NANOG, and SOX2, highlighting issues that must be considered when interpreting data about their expression on putative PSCs.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Authors. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Biochemistry and Cell Biology; Biological Sciences; Stem Cell Research - Embryonic - Non-Human; Stem Cell Research - Nonembryonic - Human; Regenerative Medicine; Stem Cell Research - Embryonic - Human; Stem Cell Research; Generic health relevance |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) > Department of Biomedical Science (Sheffield) The University of Sheffield > Faculty of Science (Sheffield) > School of Biosciences (Sheffield) |
Funding Information: | Funder Grant number MEDICAL RESEARCH COUNCIL MR/L012537/1 EUROPEAN COMMISSION - HORIZON 2020 824070 MEDICAL RESEARCH COUNCIL MR/V002163/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 19 Jan 2024 15:04 |
Last Modified: | 19 Jan 2024 15:04 |
Status: | Published |
Publisher: | Elsevier BV |
Refereed: | Yes |
Identification Number: | 10.1016/j.stemcr.2023.11.012 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:207825 |