Sharma, Rajat, Ungar, Daniel orcid.org/0000-0002-9852-6160, Dyson, Edward et al. (2 more authors) (2024) Functional magnetic nanoparticles for protein delivery applications:understanding protein-nanoparticle interactions. Nanoscale. ISSN 2040-3372
Abstract
Iron oxide nanoparticles (IONPs) surface functionalised with thermo-responsive polymers can encapsulate therapeutic proteins and release them upon heating with an alternating magnetic field above the lower critical solution temperature (LCST). In order to make this delivery system clinically-relevant, we prepared IONPs coated with poly-N-isopropylmethacrylamide (PNIPMAM), a polymer with LCST above human body temperature. The optimal polymer chain length and nanoparticle size to achieve LCST of ca. 45 °C were 19 kDa PNIPMAM and 16 nm IONPs. The PNIPMAM-coated IONPs could encapsulate a range of proteins which were released upon heating above LCST in the presence of a competitor protein or serum. A small amount of encapsulated protein leakage was observed below LCST. The efficiency of protein encapsulation and release was correlated with molecular weight and glycosylation state of the proteins. Magnetic heating resulted in a faster protein release as compared to conventional heating without significant temperature increase of the bulk solution.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2024 |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Depositing User: | Pure (York) |
Date Deposited: | 17 Jan 2024 12:00 |
Last Modified: | 14 Feb 2025 00:12 |
Published Version: | https://doi.org/10.1039/d3nr04544g |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1039/d3nr04544g |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:207823 |
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Description: Functional magnetic nanoparticles for protein delivery applications: understanding protein– nanoparticle interactions
Licence: CC-BY-NC 2.5