Bashir, N. orcid.org/0000-0002-0547-0947, Leathard, A.S. orcid.org/0000-0003-2387-1178, McHugh, M. et al. (5 more authors) (2024) On the use of modelling antagonistic enzymes to aid in temporal programming of pH and PVA–borate gelation. Molecular Systems Design & Engineering, 9 (4). pp. 372-381. ISSN 2058-9689
Abstract
Feedback through enzyme reactions creates new possibilities for the temporal programming of material properties in bioinspired applications, such as transient adhesives; however, there have been limited attempts to model such behavior. Here, we used two antagonistic enzymes, urease in watermelon seed powder and esterase, to temporally control the gelation of a poly(vinyl alcohol)–borate hydrogel in a one-pot formulation. Urease produces base (ammonia), and esterase produces acid (acetic acid), generating a pH pulse, which was coupled with reversible complexation of PVA. For improved understanding of the pulse properties and gel lifetime, the pH profile was investigated by comparison of the experiments with kinetic simulations of the enzyme reactions and relevant equilibria. The model reproduced the general trends with the initial concentrations and was used to help identify conditions for pulse-like behaviour as the substrate concentrations were varied.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Royal Society of Chemistry and IChemE. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. (http://creativecommons.org/licenses/by-nc/3.0/) |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 09 Jan 2024 10:30 |
Last Modified: | 01 Nov 2024 16:39 |
Status: | Published |
Publisher: | Royal Society of Chemistry (RSC) |
Refereed: | Yes |
Identification Number: | 10.1039/d3me00138e |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:207307 |