Gao, Z., Wang, M., Smith, A. et al. (1 more author) (Cover date: 1 December 2023) YY1 Binding to Regulatory Elements That Lack Enhancer Activity Promotes Locus Folding and Gene Activation. Journal of Molecular Biology, 435 (23). 168315. ISSN 0022-2836
Abstract
Enhancers activate their cognate promoters over huge distances but how enhancer/promoter interactions become established is not completely understood. There is strong evidence that cohesin-mediated loop extrusion is involved but this does not appear to be a universal mechanism. Here, we identify an element within the mouse immunoglobulin lambda (Igλ) light chain locus, HSCλ1, that has characteristics of active regulatory elements but lacks intrinsic enhancer or promoter activity. Remarkably, knock-out of the YY1 binding site from HSCλ1 reduces Igλ transcription significantly and disrupts enhancer/promoter interactions, even though these elements are >10 kb from HSCλ1. Genome-wide analyses of mouse embryonic stem cells identified 2671 similar YY1-bound, putative genome organizing elements that lie within CTCF/cohesin loop boundaries but that lack intrinsic enhancer activity. We suggest that such elements play a fundamental role in locus folding and in facilitating enhancer/promoter interactions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | 2023 The Author(s). This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | enhancer, promoter, locus folding, YY1 binding, gene activation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Molecular & Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 31 Oct 2023 10:24 |
Last Modified: | 31 Oct 2023 10:24 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jmb.2023.168315 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:204659 |