Sabourin, Katherine R., Mugisha, Joseph, Asiki, Gershim et al. (8 more authors) (2023) Epstein–Barr virus (EBV) antibody changes over time in a general population cohort in rural Uganda, 1992–2008. Infectious Agents and Cancer. 55. ISSN 1750-9378
Abstract
Background: Epstein–Barr virus (EBV) infection is ubiquitous and in sub-Saharan Africa, occurs early in life. In a population-based rural African cohort, we leveraged historical samples from the General Population Cohort (GPC) in Uganda to examine the epidemiology of infection with EBV over time, in the era of HIV. Methods: We used 9024 serum samples collected from the GPC in 1992, 2000, 2008, from 7576 participants across the age range (0–99 years of age) and tested for anti-EBV immunoglobulin G (IgG) antibodies to EAd, VCA, and EBNA-1 using a multiplex bead-based assay. The related gammaherpesvirus, Kaposi’s sarcoma-associated herpesvirus (KSHV) seropositivity was also determined by detection of anti-KSHV IgG antibodies to K8.1 or ORF73 measured by recombinant protein enzyme-linked immunosorbent assay. Data on sex, age, and HIV serostatus were also collected. EBV seropositivity was modeled with age (excluding those under one year, who may have had maternal antibodies), sex, HIV serostatus, and KSHV serostatus using generalized linear mixed effects models to produce beta estimates. Results: More than 93% of children were EBV seropositive by one year of age. EBV seropositivity was significantly associated with KSHV seropositivity. Anti-EBNA-1 antibody levels decreased with increasing age and were lower on average in people living with HIV. In general, anti-EAd antibody levels increased with age, were higher in males and KSHV seropositive persons, but decreased over calendar time. Anti-VCA antibody levels increased with age and with calendar time and were higher in KSHV seropositive persons but lower in males. Conclusions: This is the first study to identify factors associated with EBV antibodies across the entire life-course in rural sub-Saharan Africa. Consistent with other studies, EBV was near ubiquitous in the population by age one year. Patterns of antibodies show changes by age, sex and calendar time, but no association with HIV was evident, suggesting no relationship between EBV sero-epidemiology and the spread of HIV in the population over time in Uganda.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Funding Information: This work was supported by the Welcome Trust (Grant number: 090132) and with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261201500003I and NCI contract 75N91019D00024 to DW. The GPC is funded by the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement. The funders had no role in study design, data collection, analysis or interpretation of the data. The content of this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of NCI or NIH. Publisher Copyright: © 2023, BioMed Central Ltd., part of Springer Nature. |
Keywords: | Antibodies,Epstein–Barr virus (EBV),HIV,Kaposi’s sarcoma-associated herpesvirus (KSHV),Life course,Pre-ART,Serology,Sub-Saharan Africa,Uganda |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Health Sciences (York) |
Depositing User: | Pure (York) |
Date Deposited: | 26 Oct 2023 16:10 |
Last Modified: | 16 Oct 2024 19:33 |
Published Version: | https://doi.org/10.1186/s13027-023-00534-7 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1186/s13027-023-00534-7 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:204643 |