Bardy, Pavol orcid.org/0000-0002-1223-2584, MacDonald, Conor, Pantucek, Roman et al. (2 more authors) (2023) Jorvik: a membrane-containing phage that will likely found a new family within Vinavirales. iScience. ISSN 2589-0042
Abstract
Although membrane-containing dsDNA bacterial viruses are some of the most prevalent predators in aquatic environments, we know little about how they function due to their intractability in the laboratory. Here, we have identified and thoroughly characterized a new type of membrane-containing bacteriophage, Jorvik, that infects the freshwater mixotrophic model bacterium Rhodobacter capsulatus. Jorvik is extremely virulent, can persist in the host integrated into the RuBisCo operon and encodes two experimentally verified cell wall hydrolases. Jorvik-like prophages are abundant in the genomes of alpha-proteobacteria, are distantly related to known viruses of the class Tectiliviricetes, and we propose they should be classified as a new family. Crucially, we demonstrate how widely used phage manipulation methods should be adjusted to prevent loss of virus infectivity. Our thorough characterization of environmental phage Jorvik provides important experimental insights about phage diversity and interactions in microbial communities that are often unexplored in common metagenomic analyses.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 The Author(s) |
Keywords: | Phage,BACTERIA,Rhodobacter,Vinavirales,Membrane,ENDOLYSIN,Lysogeny,Lysis,PHYLOGENETIC ANALYSIS |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/V016288/1 WELLCOME TRUST 224067/Z/21/Z |
Depositing User: | Pure (York) |
Date Deposited: | 04 Oct 2023 11:50 |
Last Modified: | 01 Nov 2024 01:26 |
Published Version: | https://doi.org/10.1016/j.isci.2023.108104 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1016/j.isci.2023.108104 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:203948 |
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