Zulfiqar, S., Blando, F., Orfila, C. et al. (2 more authors) (Cover date: September-1 2023) Chromogenic assay is more efficient in identifying α-amylase inhibitory properties of anthocyanin-rich samples when com-pared to the 3,5-dinitrosalicylic acid (DNS) assay. Molecules, 28 (17). 6399. ISSN 1420-3049
Abstract
The inhibition of carbohydrate digestion by plant bioactive compounds is a potential dietary strategy to counteract type 2 diabetes. Indeed, inhibition of α-amylase, a key enzyme that carries out the bulk of starch digestion, has been demonstrated for a range of bioactive compounds including anthocyanins; however, sample pigmentation often interferes with measurements, affecting colorimetric assay outcomes. Therefore, the present study compared the performance of a direct chromogenic assay, using 2-chloro-4 nitrophenyl α-D-maltotrioside (CNPG3) as a substrate, with the commonly used 3,5-dinitrosalicylic acid (DNS) assay. The direct chromogenic assay demonstrated a 5–10-fold higher sensitivity to determine α-amylase inhibition in various samples, including acarbose as a reference, pure anthocyanins, and anthocyanin-rich samples. The IC50 values of acarbose presented as 37.6 μg/mL and 3.72 μg/mL for the DNS assay and the direct chromogenic assay, respectively, whereas purified anthocyanins from blackcurrant showed IC50 values of 227.4 µg/mL and 35.0 µg/mL. The direct chromogenic assay is easy to perform, fast, reproducible, and suitable for high-throughput screening of pigmented α-amylase inhibitors.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
Keywords: | α-amylase; anthocyanins; direct chromogenic assay; DNS assay; carbohydrate digestion |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Nutrition and Public Health (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 01 Sep 2023 10:26 |
Last Modified: | 27 Sep 2023 14:07 |
Published Version: | https://www.mdpi.com/1420-3049/28/17/6399 |
Status: | Published |
Publisher: | MDPI |
Identification Number: | 10.3390/molecules28176399 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:202897 |