Schulz, E.C., Yorke, B.A. orcid.org/0000-0002-1868-8435, Pearson, A.R. orcid.org/0000-0001-8499-7490 et al. (1 more author) (2022) Best practices for time-resolved serial synchrotron crystallography. Acta Crystallographica Section D Structural Biology, 78 (1). pp. 14-29. ISSN 2059-7983
Abstract
With recent developments in X-ray sources, instrumentation and data-analysis tools, time-resolved crystallographic experiments, which were originally the preserve of a few expert groups, are becoming simpler and can be carried out at more radiation sources, and are thus increasingly accessible to a growing user base. However, these experiments are just that: discrete experiments, not just `data collections'. As such, careful planning and consideration of potential pitfalls is required to enable a successful experiment. Here, some of the key factors that should be considered during the planning and execution of a time-resolved structural study are outlined, with a particular focus on synchrotron-based experiments.
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Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This article is protected by copyright. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | time-resolved crystallography; serial crystallography; SSX; structural enzymology; mechanistic structural biology |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Physical Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Jul 2023 10:57 |
Last Modified: | 17 Jul 2023 10:57 |
Status: | Published |
Publisher: | International Union of Crystallography |
Identification Number: | 10.1107/s2059798321011621 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:201580 |
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Filename: Best practices for time-resolved serial synchrotron crystallography.pdf
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