Blagova, Lena, Miller, Alex Henrique, Bennett, Megan et al. (4 more authors) (2023) Thermostable homologues of the periplasmic siderophore-binding protein CeuE from Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius. Acta Crystallographica Section D: Structural Biology. pp. 694-705. ISSN 2059-7983
Abstract
Siderophore-binding proteins from two thermophilic bacteria, Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius, were identified from a search of sequence databases, cloned and overexpressed. They are homologues of the well characterized protein CjCeuE from Campylobacter jejuni. The iron-binding histidine and tyrosine residues are conserved in both thermophiles. Crystal structures were determined of the apo proteins and of their complexes with iron(III)-azotochelin and its analogue iron(III)-5-LICAM. The thermostability of both homologues was shown to be about 20C higher than that of CjCeuE. Similarly, the tolerance of the homologues to the organic solvent dimethylformamide (DMF) was enhanced, as reflected by the respective binding constants for these ligands measured in aqueous buffer at pH 7.5 in the absence and presence of 10% and 20% DMF. Consequently, these thermophilic homologues offer advantages in the development of artificial metalloenzymes using the CeuE family.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | thermophilic proteins; siderophore binding; structure; biophysical characterization |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Chemistry (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/W011131/1 UNSPECIFIED EP/T007338/1 |
Depositing User: | Pure (York) |
Date Deposited: | 12 Jun 2023 11:50 |
Last Modified: | 27 Nov 2024 00:46 |
Published Version: | https://doi.org/10.1107/S2059798323004473 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1107/S2059798323004473 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:200339 |
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Description: Thermostable homologues of the periplasmic siderophore-binding protein CeuE from Geobacillus stearothermophilus and Parageobacillus thermoglucosidasius
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